Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V...

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Bibliographic Details
Main Authors: Hasima, N.N., Saidi, J., Thong, Kwai Lin, Ansary, A.
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2001
Subjects:
Online Access:http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf
http://eprints.um.edu.my/9969/
http://www.msmbb.my/index.php/archive-issues
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