7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation
In continuation of our interest towards the elucidation of apoptotic pathways of cytotoxic phytocompounds, we have embarked upon a study on the anticancer effects of 7 alpha-hydroxy-beta-sitosterol (CT1), a rare natural phytosterol oxide isolated from Chisocheton tomentosus. CT1 was found to be cyto...
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my.um.eprints.77832020-01-29T06:22:04Z http://eprints.um.edu.my/7783/ 7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation Tasyriq, Mohammad Najmuldeen, Ibrahim A. In, Lionel L.A. Mohamad, Khalit Awang, Khalijah Nagoor, Noor Hasima QH301 Biology In continuation of our interest towards the elucidation of apoptotic pathways of cytotoxic phytocompounds, we have embarked upon a study on the anticancer effects of 7 alpha-hydroxy-beta-sitosterol (CT1), a rare natural phytosterol oxide isolated from Chisocheton tomentosus. CT1 was found to be cytotoxic on three different human tumor cell lines with minimal effects on normal cell controls, where cell viability levels were maintained >= 80 upon treatment. Our results showed that cell death in MCF-7 breast tumor cells was achieved through the induction of apoptosis via downregulation of the ERK1/2 signaling pathway. CT1 was also found to increase proapoptotic Bax protein levels, while decreasing anti-apoptotic Bcl-2 protein levels, suggesting the involvement of the intrinsic pathway. Reduced levels of initiator procaspase-9 and executioner procaspase-3 were also observed following CT1 exposure, confirming the involvement of cytochrome c-mediated apoptosis via the mitochondrial pathway. These results demonstrated the cytotoxic and apoptotic ability of 7 alpha-hydroxy-beta-sitosterol and suggest its potential anti-cancer use particularly on breast adenocarcinoma cells. Hindawi 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/7783/1/7_alpha-Hydroxy-beta-Sitosterol_from_Chisocheton_tomentosus_Induces_Apoptosis_via_Dysregulation_of_Cell.pdf Tasyriq, Mohammad and Najmuldeen, Ibrahim A. and In, Lionel L.A. and Mohamad, Khalit and Awang, Khalijah and Nagoor, Noor Hasima (2012) 7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation. Evidence-Based Complementary and Alternative Medicine, 2012. p. 765316. ISSN 1741-427X https://doi.org/10.1155/2012/765316 doi:10.1155/2012/765316 |
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QH301 Biology Tasyriq, Mohammad Najmuldeen, Ibrahim A. In, Lionel L.A. Mohamad, Khalit Awang, Khalijah Nagoor, Noor Hasima 7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
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In continuation of our interest towards the elucidation of apoptotic pathways of cytotoxic phytocompounds, we have embarked upon a study on the anticancer effects of 7 alpha-hydroxy-beta-sitosterol (CT1), a rare natural phytosterol oxide isolated from Chisocheton tomentosus. CT1 was found to be cytotoxic on three different human tumor cell lines with minimal effects on normal cell controls, where cell viability levels were maintained >= 80 upon treatment. Our results showed that cell death in MCF-7 breast tumor cells was achieved through the induction of apoptosis via downregulation of the ERK1/2 signaling pathway. CT1 was also found to increase proapoptotic Bax protein levels, while decreasing anti-apoptotic Bcl-2 protein levels, suggesting the involvement of the intrinsic pathway. Reduced levels of initiator procaspase-9 and executioner procaspase-3 were also observed following CT1 exposure, confirming the involvement of cytochrome c-mediated apoptosis via the mitochondrial pathway. These results demonstrated the cytotoxic and apoptotic ability of 7 alpha-hydroxy-beta-sitosterol and suggest its potential anti-cancer use particularly on breast adenocarcinoma cells. |
format |
Article |
author |
Tasyriq, Mohammad Najmuldeen, Ibrahim A. In, Lionel L.A. Mohamad, Khalit Awang, Khalijah Nagoor, Noor Hasima |
author_facet |
Tasyriq, Mohammad Najmuldeen, Ibrahim A. In, Lionel L.A. Mohamad, Khalit Awang, Khalijah Nagoor, Noor Hasima |
author_sort |
Tasyriq, Mohammad |
title |
7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
title_short |
7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
title_full |
7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
title_fullStr |
7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
title_full_unstemmed |
7 alpha-Hydroxy-beta-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation |
title_sort |
7 alpha-hydroxy-beta-sitosterol from chisocheton tomentosus induces apoptosis via dysregulation of cellular bax/bcl-2 ratio and cell cycle arrest by downregulating erk1/2 activation |
publisher |
Hindawi |
publishDate |
2012 |
url |
http://eprints.um.edu.my/7783/1/7_alpha-Hydroxy-beta-Sitosterol_from_Chisocheton_tomentosus_Induces_Apoptosis_via_Dysregulation_of_Cell.pdf http://eprints.um.edu.my/7783/ https://doi.org/10.1155/2012/765316 |
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1657488187053834240 |
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13.188404 |