Cloning, Expression, and Purification of Recombinant Protein from a Single Synthetic Multivalent Construct of Mycobacterium Tuberculosis

• Main Authors: Fang, Chee Mun, Zainuddin, Zainul F., Musa, Mustaffa, Thong, Kwai Lin
• Format: Article
• Published: Elsevier 2006
• Subjects: Q Science (General); QH Natural history; QR Microbiology
• Online Access: http://eprints.um.edu.my/5485/; https://doi.org/10.1016/j.pep.2005.12.007

Tuberculosis remains a major infectious disease with over 8 million new cases and 2 million deaths annually. Therefore, a vaccine more potent than BCG is desperately needed. In this regard, an approximately 800 bp DNA encoding a mycobacterial synthetic gene designated as VacIII (containing ubiquitin...