Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a...
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my.um.eprints.459522024-11-15T01:03:29Z http://eprints.um.edu.my/45952/ Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study Windarsih, Anjar Abu Bakar, Nor Kartini Rohman, Abdul Erwanto, Yuny Q Science (General) QD Chemistry Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy ((RX)-X-2 = 0.980, and (RY)-Y-2 = 0.980) and good predictivity (Q(2) = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (% w/w) in BM with high accuracy (R-2 > 0.990). A number of metabolites, potential for biomarker candidates, were identified to differentiate BM and that adulterated with DM. It showed that the combination of a non-targeted LC-HRMS Orbitrap metabolomics and chemometrics could detect up to 0.1% w/w of DM adulteration. The developed method was successfully applied for analysis of commercial meatball samples (n = 28). Moreover, pathway analysis revealed that beta-alanine, histidine, and ether lipid metabolism were significantly affected by dog meat adulteration. In summary, this developed method has great potential to be developed and used as an alternative method for analysis of non-halal meats in halal meat products. SpringerNature 2024-03 Article PeerReviewed Windarsih, Anjar and Abu Bakar, Nor Kartini and Rohman, Abdul and Erwanto, Yuny (2024) Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study. Analytical Sciences, 40 (3, SI). pp. 385-397. ISSN 0910-6340, DOI https://doi.org/10.1007/s44211-023-00470-x <https://doi.org/10.1007/s44211-023-00470-x>. https://doi.org/10.1007/s44211-023-00470-x 10.1007/s44211-023-00470-x |
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Q Science (General) QD Chemistry Windarsih, Anjar Abu Bakar, Nor Kartini Rohman, Abdul Erwanto, Yuny Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
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Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy ((RX)-X-2 = 0.980, and (RY)-Y-2 = 0.980) and good predictivity (Q(2) = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (% w/w) in BM with high accuracy (R-2 > 0.990). A number of metabolites, potential for biomarker candidates, were identified to differentiate BM and that adulterated with DM. It showed that the combination of a non-targeted LC-HRMS Orbitrap metabolomics and chemometrics could detect up to 0.1% w/w of DM adulteration. The developed method was successfully applied for analysis of commercial meatball samples (n = 28). Moreover, pathway analysis revealed that beta-alanine, histidine, and ether lipid metabolism were significantly affected by dog meat adulteration. In summary, this developed method has great potential to be developed and used as an alternative method for analysis of non-halal meats in halal meat products. |
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Article |
author |
Windarsih, Anjar Abu Bakar, Nor Kartini Rohman, Abdul Erwanto, Yuny |
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Windarsih, Anjar Abu Bakar, Nor Kartini Rohman, Abdul Erwanto, Yuny |
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Windarsih, Anjar |
title |
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
title_short |
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
title_full |
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
title_fullStr |
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
title_full_unstemmed |
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study |
title_sort |
analysis of dog meat adulteration in beef meatballs using non-targeted uhplc-orbitrap hrms metabolomics and chemometrics for halal authentication study |
publisher |
SpringerNature |
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2024 |
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http://eprints.um.edu.my/45952/ https://doi.org/10.1007/s44211-023-00470-x |
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1816130480350691328 |
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