Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study

Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a...

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Main Authors: Windarsih, Anjar, Abu Bakar, Nor Kartini, Rohman, Abdul, Erwanto, Yuny
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Published: SpringerNature 2024
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Online Access:http://eprints.um.edu.my/45952/
https://doi.org/10.1007/s44211-023-00470-x
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spelling my.um.eprints.459522024-11-15T01:03:29Z http://eprints.um.edu.my/45952/ Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study Windarsih, Anjar Abu Bakar, Nor Kartini Rohman, Abdul Erwanto, Yuny Q Science (General) QD Chemistry Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy ((RX)-X-2 = 0.980, and (RY)-Y-2 = 0.980) and good predictivity (Q(2) = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (% w/w) in BM with high accuracy (R-2 > 0.990). A number of metabolites, potential for biomarker candidates, were identified to differentiate BM and that adulterated with DM. It showed that the combination of a non-targeted LC-HRMS Orbitrap metabolomics and chemometrics could detect up to 0.1% w/w of DM adulteration. The developed method was successfully applied for analysis of commercial meatball samples (n = 28). Moreover, pathway analysis revealed that beta-alanine, histidine, and ether lipid metabolism were significantly affected by dog meat adulteration. In summary, this developed method has great potential to be developed and used as an alternative method for analysis of non-halal meats in halal meat products. SpringerNature 2024-03 Article PeerReviewed Windarsih, Anjar and Abu Bakar, Nor Kartini and Rohman, Abdul and Erwanto, Yuny (2024) Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study. Analytical Sciences, 40 (3, SI). pp. 385-397. ISSN 0910-6340, DOI https://doi.org/10.1007/s44211-023-00470-x <https://doi.org/10.1007/s44211-023-00470-x>. https://doi.org/10.1007/s44211-023-00470-x 10.1007/s44211-023-00470-x
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Windarsih, Anjar
Abu Bakar, Nor Kartini
Rohman, Abdul
Erwanto, Yuny
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
description Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy ((RX)-X-2 = 0.980, and (RY)-Y-2 = 0.980) and good predictivity (Q(2) = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (% w/w) in BM with high accuracy (R-2 > 0.990). A number of metabolites, potential for biomarker candidates, were identified to differentiate BM and that adulterated with DM. It showed that the combination of a non-targeted LC-HRMS Orbitrap metabolomics and chemometrics could detect up to 0.1% w/w of DM adulteration. The developed method was successfully applied for analysis of commercial meatball samples (n = 28). Moreover, pathway analysis revealed that beta-alanine, histidine, and ether lipid metabolism were significantly affected by dog meat adulteration. In summary, this developed method has great potential to be developed and used as an alternative method for analysis of non-halal meats in halal meat products.
format Article
author Windarsih, Anjar
Abu Bakar, Nor Kartini
Rohman, Abdul
Erwanto, Yuny
author_facet Windarsih, Anjar
Abu Bakar, Nor Kartini
Rohman, Abdul
Erwanto, Yuny
author_sort Windarsih, Anjar
title Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
title_short Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
title_full Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
title_fullStr Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
title_full_unstemmed Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
title_sort analysis of dog meat adulteration in beef meatballs using non-targeted uhplc-orbitrap hrms metabolomics and chemometrics for halal authentication study
publisher SpringerNature
publishDate 2024
url http://eprints.um.edu.my/45952/
https://doi.org/10.1007/s44211-023-00470-x
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