RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

• Main Authors: Lai, Meng Yee, Suppiah, Jeyanthi, Thayan, Ravindran, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Lau, Yee Ling
• Format: Article
• Published: BioMed Central Ltd 2022
• Subjects: R Medicine (General); RV Botanic, Thomsonian, and eclectic medicine
• Online Access: http://eprints.um.edu.my/43188/

Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification...