Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

• Main Authors: Lai, Meng Yee, Bukhari, Fatma Diyana Mohd, Zulkefli, Nur Zulaikha, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Suppiah, Jeyanthi, Thayan, Ravindran, Isa, Mohd Khairi Mat, Wahid, Nur Zafirah Abdul, Lau, Yee Ling
• Format: Article
• Published: BMC 2022
• Subjects: R Medicine (General)
• Online Access: http://eprints.um.edu.my/41532/

Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection met...