Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl
Background Mahkota Dewa Phaleria macrocarpa (Scheff) Boerl.] fruit in vitro and in- vivo can decrease and prevent elevation of the blood pressure, lower plasma glucose levels, possess an antioxidant effect, and recover liver and kidney damage in rats. This study aimed to determine the structure and...
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my.um.eprints.386012023-07-07T03:31:40Z http://eprints.um.edu.my/38601/ Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl Eff, Aprilita Rina Yanti Huri, Hasniza Zaman Radji, Maksum Munim, Abdul Suyatna, F. D. Eden, Yonatan RS Pharmacy and materia medica Background Mahkota Dewa Phaleria macrocarpa (Scheff) Boerl.] fruit in vitro and in- vivo can decrease and prevent elevation of the blood pressure, lower plasma glucose levels, possess an antioxidant effect, and recover liver and kidney damage in rats. This study aimed to determine the structure and inhibitory activity of angiotensin-converting enzyme inhibitors (ACE) from the Mahkota Dewa fruit.Methods The fruit powder was macerated using methanol and then partitioned by hexane, ethyl acetate, n-butanol, and water. The fractions were chromatographed on the column chromatography and incorporated with TLC and recrystallization to give pure compounds. The structures of isolated compounds were determined by UV-Visible, FT-IR, MS, proton (H-1-NMR), carbon (C-13-NMR), and 2D-NMR techniques encompassing HMQC and HMBC spectra. The compounds were evaluated for their ACE inhibitory activity, and the strongest compound was determined by the kinetics enzyme inhibition.Results Based on the spectral data, the isolated compounds were determined as 6,4-dihydroxy-4-methoxybenzophenone-2-O-beta-D-glucopyranoside (1), 4,4 ` -dihydroxy-6-methoxybenzophenone-2-O-beta-D-glucopyranoside (2) and mangiferin (3). IC50 values of the isolated compounds 1, 2 and 3 were 0.055, 0.07, and 0.025 mM, respectively.Conclusion The three compounds have ACE inhibitor and mangiferin demonstrated the best ACE inhibitory activity with competitive inhibition on ACE with the type of inhibition kinetics is competitive inhibition. BMC 2023-02 Article PeerReviewed Eff, Aprilita Rina Yanti and Huri, Hasniza Zaman and Radji, Maksum and Munim, Abdul and Suyatna, F. D. and Eden, Yonatan (2023) Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl. BMC Complementary Medicine And Therapies, 23 (1). ISSN 2662-7671, DOI https://doi.org/10.1186/s12906-023-03889-x <https://doi.org/10.1186/s12906-023-03889-x>. 10.1186/s12906-023-03889-x |
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RS Pharmacy and materia medica Eff, Aprilita Rina Yanti Huri, Hasniza Zaman Radji, Maksum Munim, Abdul Suyatna, F. D. Eden, Yonatan Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
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Background Mahkota Dewa Phaleria macrocarpa (Scheff) Boerl.] fruit in vitro and in- vivo can decrease and prevent elevation of the blood pressure, lower plasma glucose levels, possess an antioxidant effect, and recover liver and kidney damage in rats. This study aimed to determine the structure and inhibitory activity of angiotensin-converting enzyme inhibitors (ACE) from the Mahkota Dewa fruit.Methods The fruit powder was macerated using methanol and then partitioned by hexane, ethyl acetate, n-butanol, and water. The fractions were chromatographed on the column chromatography and incorporated with TLC and recrystallization to give pure compounds. The structures of isolated compounds were determined by UV-Visible, FT-IR, MS, proton (H-1-NMR), carbon (C-13-NMR), and 2D-NMR techniques encompassing HMQC and HMBC spectra. The compounds were evaluated for their ACE inhibitory activity, and the strongest compound was determined by the kinetics enzyme inhibition.Results Based on the spectral data, the isolated compounds were determined as 6,4-dihydroxy-4-methoxybenzophenone-2-O-beta-D-glucopyranoside (1), 4,4 ` -dihydroxy-6-methoxybenzophenone-2-O-beta-D-glucopyranoside (2) and mangiferin (3). IC50 values of the isolated compounds 1, 2 and 3 were 0.055, 0.07, and 0.025 mM, respectively.Conclusion The three compounds have ACE inhibitor and mangiferin demonstrated the best ACE inhibitory activity with competitive inhibition on ACE with the type of inhibition kinetics is competitive inhibition. |
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Article |
author |
Eff, Aprilita Rina Yanti Huri, Hasniza Zaman Radji, Maksum Munim, Abdul Suyatna, F. D. Eden, Yonatan |
author_facet |
Eff, Aprilita Rina Yanti Huri, Hasniza Zaman Radji, Maksum Munim, Abdul Suyatna, F. D. Eden, Yonatan |
author_sort |
Eff, Aprilita Rina Yanti |
title |
Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
title_short |
Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
title_full |
Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
title_fullStr |
Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
title_full_unstemmed |
Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl |
title_sort |
angiotensin converting enzyme (ace) inhibitors activity from purified compounds fructus phaleria macrocarpa (scheff) boerl |
publisher |
BMC |
publishDate |
2023 |
url |
http://eprints.um.edu.my/38601/ |
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1772811755288788992 |
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13.160551 |