Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l

• Main Authors: Chua, Kek Heng, Kee, B., Thong, Kwai Lin
• Format: Article
• Language: English
• Published: Academic Journals 2009
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3789/1/Cloning_and_Expression_of_a_Vi_Mimotope_of_Salmonella_enterica_serovar_Typhi_through_Nucleotide-Nucleotide_Hybridization_Approach..pdf; http://eprints.um.edu.my/3789/; https://www.ajol.info/index.php/ajb/article/view/62427

A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.