Detection of Plasmodium knowlesi using recombinase polymerase amplification (RPA) combined with SYBR Green I
In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of...
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| Main Authors: | , |
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| Format: | Article |
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Elsevier
2020
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/36518/ |
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| Summary: | In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to 6 min incubation was recommended. Different sets of reaction volumes from 6.25 to 50 mu L were tested and the results indicated no differences in detection. RPA's combination with SYBR Green I is fast and easy to perform, hence this method is suitable for use in resource-limited settings. |
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