Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification

• Main Authors: Teoh, B.T., Sam, Sing Sin, Tan, K.K., Danlami, M.B., Shu, M.H., Johari, Jefree, Hooi, P.S., Brooks, D., Piepenburg, O., Nentwich, O., Wilder-Smith, A., Franco, L., Tenorio, A., AbuBakar, Sazaly
• Format: Article
• Published: American Society for Microbiology 2015
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/19565/; http://dx.doi.org/10.1128/JCM.02648-14

A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcriptionrecombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay en...