In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

Morinda elliptica L. (Rubiaceae) is a phytomedicinal herb, used to treat gastrointestinal complications in Peninsular Malaysia. The study evaluates the in vivo hepatoprotective activity of ethanolic extract of M. elliptica stem in thioacetamide (TAA) induced liver fibrosis in male Sprague Drawly rat...

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Main Authors: Bradosty, Sarwan W., Hamad, Saber W., Agha, Nabaz Fisal Shakir, Shaikh, Faiyaz Khudaboddin, Qadir Nanakali, Nadir Mustafa, Aziz, Peshawa Yunis, Salehen, Nur'Ain, Suzergoz, Faruk, Abdulla, Mahmood Ameen
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Published: Wiley 2021
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spelling my.um.eprints.341602022-06-20T06:47:27Z http://eprints.um.edu.my/34160/ In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide Bradosty, Sarwan W. Hamad, Saber W. Agha, Nabaz Fisal Shakir Shaikh, Faiyaz Khudaboddin Qadir Nanakali, Nadir Mustafa Aziz, Peshawa Yunis Salehen, Nur'Ain Suzergoz, Faruk Abdulla, Mahmood Ameen GE Environmental Sciences Q Science (General) Morinda elliptica L. (Rubiaceae) is a phytomedicinal herb, used to treat gastrointestinal complications in Peninsular Malaysia. The study evaluates the in vivo hepatoprotective activity of ethanolic extract of M. elliptica stem in thioacetamide (TAA) induced liver fibrosis in male Sprague Drawly rats. Thirty adult rats were divided into five groups of six rats each. Rats of the normal control group received intraperitoneal injections (i. p.) of vehicle 10% Tween-20, 5 ml/kg, and hepatotoxic group 200 mg/kg TAA three times per week respectively. Three supplementary groups were treated with TAA plus daily oral silymarin (50 mg/kg) or M. elliptica (250 or 500 mg/kg). After 8 weeks of treatment, all rats were sacrificed. Liver fibrosis was assessed by gross macroscopic and microscopic tissue analysis, histopathological, and biochemical analysis. The livers of the TAA treated group showed uniform coarse granules, hepatocytic necrosis with lymphocytes infiltration. Contrary, the livers of M. elliptica treated groups (250 and 500 mg/kg) were much smoother and the cell damage was much lesser. The livers of M. elliptica treated groups rats showed elevated activity of SOD and CAT with a significant decrease in MDA level at p < .0001. The level of liver damage parameters, that is, ALP, ALT, and AST, bilirubin, total protein, and albumin were restored to the normal comparable to silymarin. M. elliptica stem extract significantly promoted normal rat liver architecture with significant perfections in biochemical parameters. The molecular contents of M. elliptica with hepatoprotective influence could be discovered, is the future prospective of this study. Wiley 2021-12 Article PeerReviewed Bradosty, Sarwan W. and Hamad, Saber W. and Agha, Nabaz Fisal Shakir and Shaikh, Faiyaz Khudaboddin and Qadir Nanakali, Nadir Mustafa and Aziz, Peshawa Yunis and Salehen, Nur'Ain and Suzergoz, Faruk and Abdulla, Mahmood Ameen (2021) In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide. Environmental Toxicology, 36 (12). pp. 2404-2413. ISSN 1520-4081, DOI https://doi.org/10.1002/tox.23353 <https://doi.org/10.1002/tox.23353>. 10.1002/tox.23353
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic GE Environmental Sciences
Q Science (General)
spellingShingle GE Environmental Sciences
Q Science (General)
Bradosty, Sarwan W.
Hamad, Saber W.
Agha, Nabaz Fisal Shakir
Shaikh, Faiyaz Khudaboddin
Qadir Nanakali, Nadir Mustafa
Aziz, Peshawa Yunis
Salehen, Nur'Ain
Suzergoz, Faruk
Abdulla, Mahmood Ameen
In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
description Morinda elliptica L. (Rubiaceae) is a phytomedicinal herb, used to treat gastrointestinal complications in Peninsular Malaysia. The study evaluates the in vivo hepatoprotective activity of ethanolic extract of M. elliptica stem in thioacetamide (TAA) induced liver fibrosis in male Sprague Drawly rats. Thirty adult rats were divided into five groups of six rats each. Rats of the normal control group received intraperitoneal injections (i. p.) of vehicle 10% Tween-20, 5 ml/kg, and hepatotoxic group 200 mg/kg TAA three times per week respectively. Three supplementary groups were treated with TAA plus daily oral silymarin (50 mg/kg) or M. elliptica (250 or 500 mg/kg). After 8 weeks of treatment, all rats were sacrificed. Liver fibrosis was assessed by gross macroscopic and microscopic tissue analysis, histopathological, and biochemical analysis. The livers of the TAA treated group showed uniform coarse granules, hepatocytic necrosis with lymphocytes infiltration. Contrary, the livers of M. elliptica treated groups (250 and 500 mg/kg) were much smoother and the cell damage was much lesser. The livers of M. elliptica treated groups rats showed elevated activity of SOD and CAT with a significant decrease in MDA level at p < .0001. The level of liver damage parameters, that is, ALP, ALT, and AST, bilirubin, total protein, and albumin were restored to the normal comparable to silymarin. M. elliptica stem extract significantly promoted normal rat liver architecture with significant perfections in biochemical parameters. The molecular contents of M. elliptica with hepatoprotective influence could be discovered, is the future prospective of this study.
format Article
author Bradosty, Sarwan W.
Hamad, Saber W.
Agha, Nabaz Fisal Shakir
Shaikh, Faiyaz Khudaboddin
Qadir Nanakali, Nadir Mustafa
Aziz, Peshawa Yunis
Salehen, Nur'Ain
Suzergoz, Faruk
Abdulla, Mahmood Ameen
author_facet Bradosty, Sarwan W.
Hamad, Saber W.
Agha, Nabaz Fisal Shakir
Shaikh, Faiyaz Khudaboddin
Qadir Nanakali, Nadir Mustafa
Aziz, Peshawa Yunis
Salehen, Nur'Ain
Suzergoz, Faruk
Abdulla, Mahmood Ameen
author_sort Bradosty, Sarwan W.
title In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
title_short In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
title_full In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
title_fullStr In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
title_full_unstemmed In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide
title_sort in vivo hepatoprotective effect of morinda elliptica stem extract against liver fibrosis induced by thioacetamide
publisher Wiley
publishDate 2021
url http://eprints.um.edu.my/34160/
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score 13.187197