Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal...
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my.um.eprints.205952019-03-05T01:41:09Z http://eprints.um.edu.my/20595/ Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products Sultana, Sharmin Hossain, M.A. Motalib Sarker, Md Zaidul Islam Ali, Md. Eaqub Q Science (General) QD Chemistry Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal, Kosher and Hindu foods. Currently, several simplex and duplex PCR assays are available for the determination of gelatin origins but those methods are not only time-consuming and costly but also cannot reveal all information in a single assay platform. For the first time, we documented here a tetraplex PCR assay involving eukaryotic, fish, bovine, and porcine control for the simultaneous detection of these species by a single, easy and reliable approach. The assay was optimized under pure and mixed gelatin matrices and validated by PCR product sequencing. Finally, 38 halal branded confectionery items were screened and 33, 2 and 3 of them yielded positive bands for bovine, porcine and eukaryotes, respectively. The detection limit was 0.1 to 0.001 ng DNA extracted from pure and mixed gelatins. The study is potentially useful in monitoring and certifying halal, kosher and vegetarian issues in processed foods, pharmaceuticals and cosmetics. Elsevier 2018 Article PeerReviewed Sultana, Sharmin and Hossain, M.A. Motalib and Sarker, Md Zaidul Islam and Ali, Md. Eaqub (2018) Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products. LWT, 92. pp. 169-176. ISSN 0023-6438 https://doi.org/10.1016/j.lwt.2018.02.019 doi:10.1016/j.lwt.2018.02.019 |
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Q Science (General) QD Chemistry Sultana, Sharmin Hossain, M.A. Motalib Sarker, Md Zaidul Islam Ali, Md. Eaqub Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
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Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal, Kosher and Hindu foods. Currently, several simplex and duplex PCR assays are available for the determination of gelatin origins but those methods are not only time-consuming and costly but also cannot reveal all information in a single assay platform. For the first time, we documented here a tetraplex PCR assay involving eukaryotic, fish, bovine, and porcine control for the simultaneous detection of these species by a single, easy and reliable approach. The assay was optimized under pure and mixed gelatin matrices and validated by PCR product sequencing. Finally, 38 halal branded confectionery items were screened and 33, 2 and 3 of them yielded positive bands for bovine, porcine and eukaryotes, respectively. The detection limit was 0.1 to 0.001 ng DNA extracted from pure and mixed gelatins. The study is potentially useful in monitoring and certifying halal, kosher and vegetarian issues in processed foods, pharmaceuticals and cosmetics. |
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Article |
author |
Sultana, Sharmin Hossain, M.A. Motalib Sarker, Md Zaidul Islam Ali, Md. Eaqub |
author_facet |
Sultana, Sharmin Hossain, M.A. Motalib Sarker, Md Zaidul Islam Ali, Md. Eaqub |
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Sultana, Sharmin |
title |
Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
title_short |
Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
title_full |
Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
title_fullStr |
Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
title_full_unstemmed |
Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products |
title_sort |
multiplex pcr to discriminate bovine, porcine, and fish dna in gelatin and confectionery products |
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Elsevier |
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2018 |
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http://eprints.um.edu.my/20595/ https://doi.org/10.1016/j.lwt.2018.02.019 |
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