Amplification-free and direct fluorometric determination of telomerase activity in cell lysates using chimeric DNA-templated silver nanoclusters

• Main Authors: Lee, Shi Ting, Rahman, Ruman, Muthoosamy, Kasturi, Mohamed, Nur Aliana Hidayah, Su, XiaoDi, Tayyab, Saad, New, Siu Yee
• Format: Article
• Published: Springer Verlag (Germany) 2019
• Subjects: Q Science (General); QH Natural history; TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.um.edu.my/20040/; https://doi.org/10.1007/s00604-018-3194-7

A fluorogenic probe has been developed for determination of telomerase activity using chimeric DNA-templated silver nanoclusters (AgNCs). The formation of AgNCs was investigated before (route A) and after (route B) telomerase elongation reaction. Both routes caused selective quenching of the yellow...