Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma
Dracaena cinnabari Balf.f. is a red resin endemic to Socotra Island, Yemen. Although there have been several reports on its therapeutic properties, information on its cytotoxicity and anticancer effects is very limited. This study utilized a bioassay-guided fractionation approach to determine the cy...
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my.um.eprints.181822017-11-09T02:25:33Z http://eprints.um.edu.my/18182/ Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma Alabsi, A.M. Lim, K.L. Paterson, I.C. Ali-Saeed, R. Muharram, B.A. RK Dentistry Dracaena cinnabari Balf.f. is a red resin endemic to Socotra Island, Yemen. Although there have been several reports on its therapeutic properties, information on its cytotoxicity and anticancer effects is very limited. This study utilized a bioassay-guided fractionation approach to determine the cytotoxic and apoptosis-inducing effects of D. cinnabari on human oral squamous cell carcinoma (OSCC). The cytotoxic effects of D. cinnabari crude extract were observed in a panel of OSCC cell lines and were most pronounced in H400. Only fractions DCc and DCd were active on H400 cells; subfractions DCc15 and DCd16 exhibited the greatest cytotoxicity against H400 cells and D. cinnabari inhibited cells proliferation in a time-dependent manner. This was achieved primarily via apoptosis where externalization of phospholipid phosphatidylserine was observed using DAPI/Annexin V fluorescence double staining mechanism studied through mitochondrial membrane potential assay cytochrome c enzyme-linked immunosorbent and caspases activities revealed depolarization of mitochondrial membrane potential (MMP) and significant activation of caspases 9 and 3/7, concomitant with S phase arrest. Apoptotic proteins array suggested that MMP was regulated by Bcl-2 proteins family as results demonstrated an upregulation of Bax, Bad, and Bid as well as downregulation of Bcl-2. Hence, D. cinnabari has the potential to be developed as an anticancer agent. Hindawi Publishing Corporation 2016 Article PeerReviewed Alabsi, A.M. and Lim, K.L. and Paterson, I.C. and Ali-Saeed, R. and Muharram, B.A. (2016) Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International, 2016. p. 4904016. ISSN 2314-6133 http://dx.doi.org/10.1155/2016/4904016 doi:10.1155/2016/4904016 |
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RK Dentistry Alabsi, A.M. Lim, K.L. Paterson, I.C. Ali-Saeed, R. Muharram, B.A. Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
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Dracaena cinnabari Balf.f. is a red resin endemic to Socotra Island, Yemen. Although there have been several reports on its therapeutic properties, information on its cytotoxicity and anticancer effects is very limited. This study utilized a bioassay-guided fractionation approach to determine the cytotoxic and apoptosis-inducing effects of D. cinnabari on human oral squamous cell carcinoma (OSCC). The cytotoxic effects of D. cinnabari crude extract were observed in a panel of OSCC cell lines and were most pronounced in H400. Only fractions DCc and DCd were active on H400 cells; subfractions DCc15 and DCd16 exhibited the greatest cytotoxicity against H400 cells and D. cinnabari inhibited cells proliferation in a time-dependent manner. This was achieved primarily via apoptosis where externalization of phospholipid phosphatidylserine was observed using DAPI/Annexin V fluorescence double staining mechanism studied through mitochondrial membrane potential assay cytochrome c enzyme-linked immunosorbent and caspases activities revealed depolarization of mitochondrial membrane potential (MMP) and significant activation of caspases 9 and 3/7, concomitant with S phase arrest. Apoptotic proteins array suggested that MMP was regulated by Bcl-2 proteins family as results demonstrated an upregulation of Bax, Bad, and Bid as well as downregulation of Bcl-2. Hence, D. cinnabari has the potential to be developed as an anticancer agent. |
format |
Article |
author |
Alabsi, A.M. Lim, K.L. Paterson, I.C. Ali-Saeed, R. Muharram, B.A. |
author_facet |
Alabsi, A.M. Lim, K.L. Paterson, I.C. Ali-Saeed, R. Muharram, B.A. |
author_sort |
Alabsi, A.M. |
title |
Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
title_short |
Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
title_full |
Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
title_fullStr |
Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
title_full_unstemmed |
Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma |
title_sort |
cell cycle arrest and apoptosis induction via modulation of mitochondrial integrity by bcl-2 family members and caspase dependence in dracaena cinnabari -treated h400 human oral squamous cell carcinoma |
publisher |
Hindawi Publishing Corporation |
publishDate |
2016 |
url |
http://eprints.um.edu.my/18182/ http://dx.doi.org/10.1155/2016/4904016 |
_version_ |
1643690633098952704 |
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13.160551 |