Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice

• Main Authors: Mathenge, E.G.M., Parquet, M.D.C., Funakoshi, Y., Houhara, S., Wong, P.F., Ichinose, A., Hasebe, F., Inoue, S., Morita, K.
• Format: Article
• Published: Society for General Microbiology 2004
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/13172/; http://vir.sgmjournals.org/content/85/9/2503.long; http://dx.doi.org/10.1099/vir.0.80120-0

The first flavivirus chimera encoding dengue 4 virus (D4) PrM and E structural proteins in a Japanese encephalitis virus (JEV) backbone was successfully generated using the long-PCR based cDNA-fragment stitching (LPCRcFS) technique, demonstrating the technique's applicability for rapid preparat...