Exochelin production in Mycobacterium neoaurum.
Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most my...
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my.um.eprints.10812017-07-08T05:42:28Z http://eprints.um.edu.my/1081/ Exochelin production in Mycobacterium neoaurum. Chan, K. RB Pathology Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated. MDPI 2009-01 Article PeerReviewed Chan, K. (2009) Exochelin production in Mycobacterium neoaurum. International Journal of Molecular Sciences, 10 (1). pp. 345-53. ISSN 1661-6596 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/ 19333449 |
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RB Pathology Chan, K. Exochelin production in Mycobacterium neoaurum. |
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Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated. |
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Article |
author |
Chan, K. |
author_facet |
Chan, K. |
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Chan, K. |
title |
Exochelin production in Mycobacterium neoaurum. |
title_short |
Exochelin production in Mycobacterium neoaurum. |
title_full |
Exochelin production in Mycobacterium neoaurum. |
title_fullStr |
Exochelin production in Mycobacterium neoaurum. |
title_full_unstemmed |
Exochelin production in Mycobacterium neoaurum. |
title_sort |
exochelin production in mycobacterium neoaurum. |
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MDPI |
publishDate |
2009 |
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http://eprints.um.edu.my/1081/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/ |
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1643686673322606592 |
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13.18916 |