Sensitivity of consumer antibacterial and non-antibacterial body washes against bacterial skin pathogens / Khairatul Ayyun Mohd Ramli

• Main Author: Mohd Ramli, Khairatul Ayyun
• Format: Thesis
• Language: English
• Published: 2019
• Subjects: Organic substances; Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene; RL Dermatology; Pathological anatomy
• Online Access: https://ir.uitm.edu.my/id/eprint/51082/1/51082.pdf; https://ir.uitm.edu.my/id/eprint/51082/

Consumer antibacterial and non-antibacterial body washes namely Dettol, Lifebuoy, T3, Lux, Shokubutsu and Palmolive were investigated for their bactericidal activities against bacterial skin pathogens, which were Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa. Gentamicin 10µg was used as control. Both types of body wash were prepared by diluting into 1:1, 1:2, 1:4 and 1:8 concentrations, and were impregnated into filter paper discs. The bactericidal activities of the body washes were evaluated using the discs diffusion method. The inhibitory zones were recorded in millimeter (mm). The data shown represent the means of the experiment performed in triplicate. Among the antibacterial body washes, T3 was able to inhibit the growth of S. aureus, S. pyogenes, and E. coli at all concentrations. Dettol was able to inhibit the growth of S. aureus and S. pyogenes at all concentrations. Lifebuoy can only inhibit the growth of S. pyogenes at all concentrations. P. aeruginosa was resistant towards all of these antibacterial body washes at any concentrations. Among the non-antibacterial body washes, Palmolive showed bactericidal activity at 1:1 and 1:2 concentrations by inhibiting the growth of S. aureus and S. pyogenes. Lux and Shokubutsu showed inhibition zones at 1:1 and 1:2 concentrations against S. pyogenes. E. coli and P. aeruginosa were not affected at all by these non-antibacterial body washes at any concentrations. In comparing both types of body washes, the significant differences (P=0.00) were observed for the inhibition zone produced among the pathogens. T3 body wash exhibited the highest bactericidal activity (19.31±13.5 mm) while Lux showed the least zone of inhibition (1.15±3.09 mm). P. aeruginosa showed the highest resistance (0.00±0.00 mm), while S. pyogenes was the most sensitive (9.33±6.16 mm) towards body washes tested. Complete resistance was shown by some of the bacterial strains even at the highest concentration of the antibacterial body wash. The non-antibacterial body wash showed satisfactory bactericidal activity on the tested pathogens. The findings suggest that non-antibacterial body wash is recommended in daily usage as the prolonged use of antibacterial agents lead to antimicrobial resistance.