Optimizing the magnesium chloride concentration in a polymerase chain reaction / Sazaliza Mat Isa

• Main Author: Mat Isa, Sazaliza
• Format: Student Project
• Language: English
• Published: 2007
• Subjects: Chemical engineering; Chemicals
• Online Access: http://ir.uitm.edu.my/id/eprint/47508/1/47508.pdf; http://ir.uitm.edu.my/id/eprint/47508/

The polymerase chain reaction (PCR) is any enzymatic method of synthesizing or amplification of large quantities of a targeted region of DNA in vitro. When developing a protocol for PCR amplification of a new DNA target, it may be important to optimize the reagent concentrations, cycling temperature...