Optimizing the magnesium chloride concentration in a polymerase chain reaction / Sazaliza Mat Isa

• المؤلف الرئيسي: Mat Isa, Sazaliza
• التنسيق: Student Project
• اللغة: English
• منشور في: 2007
• الموضوعات: Chemical engineering; Chemicals
• الوصول للمادة أونلاين: http://ir.uitm.edu.my/id/eprint/47508/1/47508.pdf; http://ir.uitm.edu.my/id/eprint/47508/

The polymerase chain reaction (PCR) is any enzymatic method of synthesizing or amplification of large quantities of a targeted region of DNA in vitro. When developing a protocol for PCR amplification of a new DNA target, it may be important to optimize the reagent concentrations, cycling temperature...