Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype
Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation usi...
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Main Authors: | , , |
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Format: | Article |
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Wiley-VCH Verlag
2017
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Online Access: | http://eprints.sunway.edu.my/494/ |
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Summary: | Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexinVimmunostaining. Overall,thesefindingssuggesttheexistenceofapoptosis inA.castellaniipossiblymediatedbyintrinsicapoptoticcascade.Furtherresearchin this field could provide avenues to selectively induce apoptosis in A. castellanii by triggering intrinsic apoptotic cascade. |
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