Scanning and transmission electron microscope changes in liver sinusoidal endothelial cells of monosodium methylarsonate (MSMA)-exposed rats

• Main Authors: Saharudin, Shahida, Buyong, Zunariah, Wan Salleh, Wan Muhamad Salahudin, Abdullah, Nor Zamzila, A.Talib, Norlelawati, Ab Rahman, Jamalludin, Abd. Fuaat, Azliana
• Format: Conference or Workshop Item
• Language: English; English; English; English
• Published: 2021
• Subjects: R Medicine (General); RB Pathology; RZ Other systems of medicine
• Online Access: http://irep.iium.edu.my/95867/1/SCMSM%202021_Book%20cover.pdf; http://irep.iium.edu.my/95867/2/SCMSM%202021_schedule.pdf; http://irep.iium.edu.my/95867/3/SCMSM%202021_abstract%20page.pdf; http://irep.iium.edu.my/95867/4/SCMSM21_e-poster%20PDF%20%28LP011%29.pdf; http://irep.iium.edu.my/95867/

Liver sinusoidal endothelial cells (LSEC) are the most abundant non-parenchymal hepatic cell population. Inorganic arsenic has been shown to cause LSEC structural and phenotypic disturbances that marked the early pathogenic changes of the liver. Monosodium methylarsonate (MSMA) is an organic-arsenic based herbicide that is used in certain part of the country as it has been thought to be less dangerous than inorganic. This study investigated the effect of MSMA on the morphological changes of the LSEC. Twenty- four male Sprague Dawley rats were divided into two groups according to duration of exposure, 2 and 6 months. Each with its corresponding control groups. MSMA was given at 63.20 mg/kg daily through oral gavage. At the end of the duration, liver tissues were harvested and processed for scanning and transmission electron microscopy. In 2-month and 6-month control rats, plates of hepatocytes were clearly seen separated by sinusoids. Sinusoids were lined by LSEC which were characterized by flattened cells, attenuated cytoplasm and the presence of numerous fenestrae arranged in groups. Groups of fenestrae on LSEC in 2-month exposed rats were present but appeared lesser that its control. While, groups of fenestrae in 6-month exposed rats were also lesser than its control with more obvious gaps seen. Chromatin condensation of LSEC were noted with few caveolae within the cytoplasm. Noticeable defenestration suggested that MSMA may lead to LSEC structural disturbances that could be the initial events in the liver pathogenic changes. Therefore, we concluded that MSMA may possess potential toxic elements as inorganic arsenic.