Liver Sinusoidal Endothelial Cell (LSEC) isolation following a liver perfusion technique

• Main Authors: Saharudin, Shahida, A. Talib, Norlelawati, Abdullah, Nor Zamzila, Ab Rahman, Jamalludin, Buyong, Zunariah
• Format: Article
• Language: English
• Published: Kulliyyah of Medicine, International Islamic University Malaysia 2017
• Subjects: R Medicine (General)
• Online Access: http://irep.iium.edu.my/62377/1/62377_Liver%20Sinusoidal%20Endothelial%20Cell%20%28LSEC%29.pdf; http://irep.iium.edu.my/62377/; http://iiumedic.net/imjm/v1/volume-16-supplementary-issue-no-1/

Introduction: Liver perfusion has been the standard method to digest and isolate liver cells including liver sinusoidal endothelial cells (LSEC). Poor cannulating skills through portal vein results in a waste of animal resource. Familiarization of both liver perfusion technique and adhering strictly...