Optimization of supercritical fluid extraction of asiaticoside from Centella Asiatica using Central Composite Design (CCD)

• Main Authors: Ruslan, Fatin Shazwani, Darnis, Deny Susanti, Taher, Muhammad, Mohammad, Nurul Farahain
• Format: Article
• Language: English
• Published: Taylor and Francis 2020
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/84851/1/Optimization%20of%20supercritical%20fluid%20extraction%20of%20asiaticoside%20from%20Centella%20asiatica%20using%20Central%20Composite%20Design%20CCD.pdf; http://irep.iium.edu.my/84851/; https://www.tandfonline.com/doi/full/10.1080/01496395.2020.1845208

The extraction of asiaticoside was performed using supercritical fluid extraction to produce highly concentrated extracts. The effects of three factors (co-solvent percentage, pressure, and tempera- ture) on the asiaticoside content were screened using the Two-Level Factorial design and optimized using the Central Composite Design. Asiaticoside was extracted following the treatment combina- tions of three parameters, namely co-solvent (5–15%), temperature (70–90°C), and pressure (1–20 MPa). The optimized extraction parameters were identified at 10%, 10.5 MPa, and 80°C for co- solvent percentage, pressure, and temperature, respectively.