Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus heterophyllus) seed that specifically recognizes and binds reversibly to galactose. Conventionally, purification of jacalin is carried out using the tedious and costly chromatographic techniques. In this study, e...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Conference or Workshop Item |
Language: | English English |
Published: |
Institute of Physics Publishing
2020
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/83698/1/83698_Response%20surface%20optimization_conf.%20article.pdf http://irep.iium.edu.my/83698/2/83698_Response%20surface%20optimization_scopus.pdf http://irep.iium.edu.my/83698/ https://iopscience.iop.org/article/10.1088/1757-899X/716/1/012019/pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.iium.irep.83698 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.836982020-11-09T06:30:48Z http://irep.iium.edu.my/83698/ Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi R Medicine (General) RS Pharmacy and materia medica T Technology (General) TD878 Special types of pollution Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus heterophyllus) seed that specifically recognizes and binds reversibly to galactose. Conventionally, purification of jacalin is carried out using the tedious and costly chromatographic techniques. In this study, extraction of jacalin from jackfruit seed crude extract were done using the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-based reverse micellar system. Reverse micellar extraction is an attractive alternative for downstream processing of various proteins. A successful reverse micellar extraction consists of two basic steps: forward and backward extraction. Forward extraction transfers a target protein from an aqueous solution into the reverse micellar solution, while backward extraction releases the protein from the reverse micelles structure into a new aqueous solution. The effects of the aqueous phase pH, NaCl concentration and AOT concentration on the forward extraction efficiency (FEE) are investigated using the response surface methodology (Box-Behnken Design). The main effects and interactions of the parameters are analyzed through the 3D surface plots. The optimum conditions for forward extraction were determined as follows: aqueous phase pH 4.58, 125 mM NaCl and 40 mM AOT. Under the optimal conditions, the FEE reached 88.04±1.30%, closer to 87.99% predicted by the model. The results indicated that AOT/isooctane reverse micelle system is effective in extracting jacalin from the jackfruit seed crude extract and verified the practicability of the BBD model for optimizing the main parameters in the forward extraction of jacalin. Institute of Physics Publishing 2020-02-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/83698/1/83698_Response%20surface%20optimization_conf.%20article.pdf application/pdf en http://irep.iium.edu.my/83698/2/83698_Response%20surface%20optimization_scopus.pdf Sy Mohamad, Sharifah Fathiyah and Mohd Said, Farhan and Abdul Munaim, Mimi Sakinah and Mohamad, Shahril and Wan Sulaiman, Wan Mohd. Azizi (2020) Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. In: 3rd Bioprocessing and Biomanufacturing Symposium 2019 (BBS 2019), 9th-10th April 2019, Lumut, Perak. https://iopscience.iop.org/article/10.1088/1757-899X/716/1/012019/pdf 10.1088/1757-899X/716/1/012019 |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English English |
topic |
R Medicine (General) RS Pharmacy and materia medica T Technology (General) TD878 Special types of pollution |
spellingShingle |
R Medicine (General) RS Pharmacy and materia medica T Technology (General) TD878 Special types of pollution Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
description |
Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus
heterophyllus) seed that specifically recognizes and binds reversibly to galactose.
Conventionally, purification of jacalin is carried out using the tedious and costly
chromatographic techniques. In this study, extraction of jacalin from jackfruit seed crude
extract were done using the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-based reverse
micellar system. Reverse micellar extraction is an attractive alternative for downstream
processing of various proteins. A successful reverse micellar extraction consists of two basic
steps: forward and backward extraction. Forward extraction transfers a target protein from an
aqueous solution into the reverse micellar solution, while backward extraction releases the
protein from the reverse micelles structure into a new aqueous solution. The effects of the
aqueous phase pH, NaCl concentration and AOT concentration on the forward extraction
efficiency (FEE) are investigated using the response surface methodology (Box-Behnken
Design). The main effects and interactions of the parameters are analyzed through the 3D
surface plots. The optimum conditions for forward extraction were determined as follows:
aqueous phase pH 4.58, 125 mM NaCl and 40 mM AOT. Under the optimal conditions, the
FEE reached 88.04±1.30%, closer to 87.99% predicted by the model. The results indicated that
AOT/isooctane reverse micelle system is effective in extracting jacalin from the jackfruit seed
crude extract and verified the practicability of the BBD model for optimizing the main
parameters in the forward extraction of jacalin. |
format |
Conference or Workshop Item |
author |
Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi |
author_facet |
Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi |
author_sort |
Sy Mohamad, Sharifah Fathiyah |
title |
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
title_short |
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
title_full |
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
title_fullStr |
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
title_full_unstemmed |
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system |
title_sort |
response surface optimization of the forward extraction of jacalin from jackfruit seeds using aot/isooctane reverse micellar system |
publisher |
Institute of Physics Publishing |
publishDate |
2020 |
url |
http://irep.iium.edu.my/83698/1/83698_Response%20surface%20optimization_conf.%20article.pdf http://irep.iium.edu.my/83698/2/83698_Response%20surface%20optimization_scopus.pdf http://irep.iium.edu.my/83698/ https://iopscience.iop.org/article/10.1088/1757-899X/716/1/012019/pdf |
_version_ |
1683230361129058304 |
score |
13.18916 |