Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis
Ethnopharmacological relevance: Porcupine bezoar (PB) is used as folk medicine for various medical conditions including cancer treatment in Malaysia. However, its toxicity profile has never been thoroughly ascertained to confirm its safe nature as an efficacious traditional medicine in the treatme...
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Online Access: | http://irep.iium.edu.my/83018/1/2020_Alaina_JO%20Ethnopharmacology%20Published.pdf http://irep.iium.edu.my/83018/7/83018_Determination%20toxic%20effects%20of%20Hystrix%20Brachyura%20Bezoar%20extracts%20using%20cancer%20cell%20lines%20and%20embryo%20zebrafish%20%28Danio%20rerio%29%20models%20and%20identification%20of%20active%20principles%20through%20GC-MS%20analysis.pdf http://irep.iium.edu.my/83018/ https://www.sciencedirect.com/science/article/abs/pii/S0378874120330191 |
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my.iium.irep.830182021-01-06T04:27:47Z http://irep.iium.edu.my/83018/ Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis Firus Khan, Al’aina Yuhainis Ahmed, Qamar Uddin Nippur, Tanzina Sharmin Hilles, Ayah K. Jalal, Tara Lay, Kek Teh Salleh, Mohd Zaki Mohd Noor, Suzita Seeni, Azman Khatib, Alfi Abdul Wahab, Ridhwan QD Chemistry Ethnopharmacological relevance: Porcupine bezoar (PB) is used as folk medicine for various medical conditions including cancer treatment in Malaysia. However, its toxicity profile has never been thoroughly ascertained to confirm its safe nature as an efficacious traditional medicine in the treatment of cancer as well as other ailments. Aim of the study: This study was aimed to reveal three different PBs’ aqueous extracts(viz. PB-A, PB-B, PB-C) chemical constituent’s profile using GC-MS analysis, anticancer property on A375, HeLa and MCF7 cancer cells, toxicity profile on zebrafish embryo morphology, EC50, LC50 and teratogenicity index. Materials and methods: PBs’ extracts characterization was performed through GC-MS analysis, in vitro anticancer effect was carried out on A375, HeLa and MCF7 cancer cell lines and finally and toxicity properties on three different PBs aqueous extracts (viz. PB-A, PB-B, PB-C) were determined using zebrafish embryo model. Results: The GC-MS analysis revealed 10 similar compounds in all PBs’ extracts. Dilauryl thiodipropionate was found to be a major compound in all PBs’ extracts followed by tetradecanoic acid. An in vitro anticancer study revealed PB extracts exerted median inhibition concentration (IC50) <50 μg/mL, on cancer cells viz. A375, HeLa and MCF7 with no significant toxicity on normal cells viz. NHDF cells. In vivo toxicity of PBs extracts found affecting tail detachment, hatching, craniofacial, brain morphology, soft tissues, edema, spinal, somites, notochord and cardiovascular system (brachycardia, disruption of blood circulation) deformities. The LC50 and EC50 demonstrated PB extracts effect as dose and time dependent with median concentration <150.0 μg/mL. Additionally, teratogenicity index (TI) viz. >1.0 revealed teratogenic property for PB extracts. Conclusions: The findings revealed that all three PBs aqueous extracts possessed anticancer activity and exhibited significant toxicological effects on zebrafish embryos with high teratogenicity index. Hence, its use as an anticancer agent requires further investigation and medical attentions to determine its safe dose. Elsevier 2020-07-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/83018/1/2020_Alaina_JO%20Ethnopharmacology%20Published.pdf application/pdf en http://irep.iium.edu.my/83018/7/83018_Determination%20toxic%20effects%20of%20Hystrix%20Brachyura%20Bezoar%20extracts%20using%20cancer%20cell%20lines%20and%20embryo%20zebrafish%20%28Danio%20rerio%29%20models%20and%20identification%20of%20active%20principles%20through%20GC-MS%20analysis.pdf Firus Khan, Al’aina Yuhainis and Ahmed, Qamar Uddin and Nippur, Tanzina Sharmin and Hilles, Ayah and K. Jalal, Tara and Lay, Kek Teh and Salleh, Mohd Zaki and Mohd Noor, Suzita and Seeni, Azman and Khatib, Alfi and Abdul Wahab, Ridhwan (2020) Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis. Journal of Ethnopharmacology, 262. pp. 1-13. ISSN 0378-8741 E-ISSN 1872-7573 https://www.sciencedirect.com/science/article/abs/pii/S0378874120330191 |
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QD Chemistry Firus Khan, Al’aina Yuhainis Ahmed, Qamar Uddin Nippur, Tanzina Sharmin Hilles, Ayah K. Jalal, Tara Lay, Kek Teh Salleh, Mohd Zaki Mohd Noor, Suzita Seeni, Azman Khatib, Alfi Abdul Wahab, Ridhwan Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
description |
Ethnopharmacological relevance: Porcupine bezoar (PB) is used as folk medicine for various medical conditions
including cancer treatment in Malaysia. However, its toxicity profile has never been thoroughly ascertained to
confirm its safe nature as an efficacious traditional medicine in the treatment of cancer as well as other ailments.
Aim of the study: This study was aimed to reveal three different PBs’ aqueous extracts(viz. PB-A, PB-B, PB-C)
chemical constituent’s profile using GC-MS analysis, anticancer property on A375, HeLa and MCF7 cancer
cells, toxicity profile on zebrafish embryo morphology, EC50, LC50 and teratogenicity index.
Materials and methods: PBs’ extracts characterization was performed through GC-MS analysis, in vitro anticancer
effect was carried out on A375, HeLa and MCF7 cancer cell lines and finally and toxicity properties on three
different PBs aqueous extracts (viz. PB-A, PB-B, PB-C) were determined using zebrafish embryo model.
Results: The GC-MS analysis revealed 10 similar compounds in all PBs’ extracts. Dilauryl thiodipropionate was
found to be a major compound in all PBs’ extracts followed by tetradecanoic acid. An in vitro anticancer study
revealed PB extracts exerted median inhibition concentration (IC50) <50 μg/mL, on cancer cells viz. A375, HeLa
and MCF7 with no significant toxicity on normal cells viz. NHDF cells. In vivo toxicity of PBs extracts found
affecting tail detachment, hatching, craniofacial, brain morphology, soft tissues, edema, spinal, somites, notochord
and cardiovascular system (brachycardia, disruption of blood circulation) deformities. The LC50 and EC50
demonstrated PB extracts effect as dose and time dependent with median concentration <150.0 μg/mL. Additionally,
teratogenicity index (TI) viz. >1.0 revealed teratogenic property for PB extracts.
Conclusions: The findings revealed that all three PBs aqueous extracts possessed anticancer activity and exhibited
significant toxicological effects on zebrafish embryos with high teratogenicity index. Hence, its use as an anticancer
agent requires further investigation and medical attentions to determine its safe dose. |
format |
Article |
author |
Firus Khan, Al’aina Yuhainis Ahmed, Qamar Uddin Nippur, Tanzina Sharmin Hilles, Ayah K. Jalal, Tara Lay, Kek Teh Salleh, Mohd Zaki Mohd Noor, Suzita Seeni, Azman Khatib, Alfi Abdul Wahab, Ridhwan |
author_facet |
Firus Khan, Al’aina Yuhainis Ahmed, Qamar Uddin Nippur, Tanzina Sharmin Hilles, Ayah K. Jalal, Tara Lay, Kek Teh Salleh, Mohd Zaki Mohd Noor, Suzita Seeni, Azman Khatib, Alfi Abdul Wahab, Ridhwan |
author_sort |
Firus Khan, Al’aina Yuhainis |
title |
Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
title_short |
Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
title_full |
Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
title_fullStr |
Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
title_full_unstemmed |
Determination toxic effects of Hystrix Brachyura Bezoar extracts using cancer cell lines and embryo zebrafish (Danio rerio) models and identification of active principles through GC-MS analysis |
title_sort |
determination toxic effects of hystrix brachyura bezoar extracts using cancer cell lines and embryo zebrafish (danio rerio) models and identification of active principles through gc-ms analysis |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://irep.iium.edu.my/83018/1/2020_Alaina_JO%20Ethnopharmacology%20Published.pdf http://irep.iium.edu.my/83018/7/83018_Determination%20toxic%20effects%20of%20Hystrix%20Brachyura%20Bezoar%20extracts%20using%20cancer%20cell%20lines%20and%20embryo%20zebrafish%20%28Danio%20rerio%29%20models%20and%20identification%20of%20active%20principles%20through%20GC-MS%20analysis.pdf http://irep.iium.edu.my/83018/ https://www.sciencedirect.com/science/article/abs/pii/S0378874120330191 |
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13.160551 |