Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis

• Main Authors: Bakar, Nur Husnina Iffah, Lee, Thong Chuan, Mohd-Hairul, Ab Rahim, Ahmad Raus, Raha, Basri, Dayang Fredalina, Adzahar, Noor Suhana
• Format: Book Chapter
• Language: English; English
• Published: Trans Tech Publications Ltd 2020
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf; http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf; http://irep.iium.edu.my/82587/; https://www.scientific.net/MSF.981.265

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.