Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL...
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| Main Authors: | , , , , , |
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| Format: | Book Chapter |
| Language: | English English |
| Published: |
Trans Tech Publications Ltd
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf http://irep.iium.edu.my/82587/ https://www.scientific.net/MSF.981.265 |
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| Summary: | A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. |
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