Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats

• Main Authors: Abdel-Wahhab, Mosaad Attia, El-Nekeety, Aziza Abdel Salam, Hathout, Amal S., Salman, Asmaa S., Abdel-Aziem, Sekena H., Sabry, Bassem A., Hassan, Nabila Salah, Abdel-Aziz, Mohammed S., Aly, Soher E., Jaswir, Irwandi
• Format: Article
• Language: English; English
• Published: Elsevier Ltd 2020
• Subjects: QH301 Biology; TP Chemical technology; TP368 Food processing and manufacture
• Online Access: http://irep.iium.edu.my/82209/2/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_FULL%20TEXT.pdf; http://irep.iium.edu.my/82209/1/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_SCOPUS.pdf; http://irep.iium.edu.my/82209/; https://www.sciencedirect.com/science/article/abs/pii/S004101012030221X

This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were treated orally for 4 weeks included the control group, AFB1-treated group (80 μg/kg b.w); fungal extract (FE)-treated groups at low (140) or high dose (280) mg/kg b.w and the groups treated with AFB1 plus FE at the two tested doses. The GC-MS analysis identified 26 compounds. The major compounds found were 1,2,3,4,6-Penta-trimethylsilyl Glucopyranose, Fmoc-L-3-(2-Naphthyl)-alanine, D-(-)-Fructopyranose, pentakis (trimethylsilyl) ether, bis (2-ethylhexyl) phthalate, trimethylsilyl ether-glucitol, and octadecanamide, N-(2- methylpropyl)-N-nitroso. The in vivo results showed that AFB1 significantly increased serum ALT, AST, creatinine, uric acid, urea, cholesterol, triglycerides, LDL, carcinoembryonic antigen, alpha-fetoprotein, interleukin-6, Malondialdehyde, nitric oxide, Bax, caspase-3 and P53 mRNA expression, chromosomal aberrations and DNA fragmentation. It decreased serum TP, albumin, HDL, Bcl-2 mRNA expression, hepatic and renal TAC, SOD and GPx content and induced histological changes in the liver and kidney. FE prevented these disturbances in a dosage-dependent manner. It could be concluded that A. niger SH2-EGY extract is safe a promising agent for pharmaceutical and food industries.