Study of cells attachment using impedance spectroscopy technique

• Main Authors: Nordin, Anis Nurashikin, S.M., Arifuzzaman, Mel, Maizirwan, Spray, David, Voiculescu, Ioana
• Format: Conference or Workshop Item
• Language: English
• Published: 2011
• Subjects: TA164 Bioengineering; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf; http://irep.iium.edu.my/8147/; http://www.asmeconferences.org/Congress2011/

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package.