MTT assay technique
Measurement of cell viability & proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. -MTT assay was the first widely accepted method that replaced the radioactive tritiated thymidine incorporation assay to measure cell proliferation -U...
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| Format: | Conference or Workshop Item |
| Language: | English English English English |
| Published: |
2018
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| Online Access: | http://irep.iium.edu.my/68829/2/CCW_Booklet_Outerpart%20F.jpg http://irep.iium.edu.my/68829/3/CCW_Booklet_Innerpart%20F.jpg http://irep.iium.edu.my/68829/4/MTT%20assay%20technique%20Dr.%20Ashour%20F.pdf http://irep.iium.edu.my/68829/1/Appointment%20Letter%20for%20Dr%20Ashour%20as%20a%20chairman%20of%20Cell%20Culture%20Workshop.pdf http://irep.iium.edu.my/68829/ |
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| Summary: | Measurement of cell viability & proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors.
-MTT assay was the first widely accepted method that replaced the radioactive tritiated thymidine incorporation assay to measure cell proliferation
-Use of the MTT tetrazolium compound to measure the number of viable cells in culture was first described by Mosmann in 1983, with the modifications suggested by Denizot & Lang in 1986 |
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