A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system

• Main Authors: Babar, Z.M., Wan Sulaiman, Wan Mohd. Azizi, Arief Ichwan, Solachuddin Jauhari, Ahmed, Qamar Uddin, Azad, Abul Kalam, Mawa, Imranul
• Format: Article
• Language: English; English; English
• Published: Taylor and Francis 2018
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/65108/16/65108-A%20simple%20method%20for%20extracting%20both%20active%20oily%20-article%20for%20myra.pdf; http://irep.iium.edu.my/65108/9/65108%20A%20simple%20method%20for%20extracting%20both%20active%20oily%20and%20water%20soluble%20extract%20SCOPUS-in-press.pdf; http://irep.iium.edu.my/65108/17/65108_A%20simple%20method%20for%20extracting%20both%20active%20oily_wos.pdf; http://irep.iium.edu.my/65108/; https://www.tandfonline.com/doi/abs/10.1080/14786419.2018.1493587

The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems.