Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture

Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by...

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Main Authors: Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Arifin, Mohd Azmir, Mel, Maizirwan, Mohd Salleh, Hamzah, Sopyan, Iis
Format: Conference or Workshop Item
Language:English
English
English
Published: Institute of Physics Publishing 2018
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Online Access:http://irep.iium.edu.my/59734/6/59734-Surface%20Modification%20of%20Polycaprolactone.pdf
http://irep.iium.edu.my/59734/12/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29.pdf
http://irep.iium.edu.my/59734/13/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29_SCOPUS.pdf
http://irep.iium.edu.my/59734/
http://iopscience.iop.org/article/10.1088/1757-899X/290/1/012016
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spelling my.iium.irep.597342019-10-23T00:39:22Z http://irep.iium.edu.my/59734/ Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Q Science (General) Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 µg/g on UV/O3 treated microcarriers as compared to the untreated (320 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93o – 49.34o) after UV/O3 treatment and subsequently after immobilisation of gelatin. The attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL microcarrier. Institute of Physics Publishing 2018-01-30 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59734/6/59734-Surface%20Modification%20of%20Polycaprolactone.pdf application/pdf en http://irep.iium.edu.my/59734/12/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29.pdf application/pdf en http://irep.iium.edu.my/59734/13/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29_SCOPUS.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd Salleh, Hamzah and Sopyan, Iis (2018) Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture. In: International Conference on Advances in Manufacturing and Materials Engineering 2017 (ICAMME17), 8th-9th August 2017, Kuala Lumpur. http://iopscience.iop.org/article/10.1088/1757-899X/290/1/012016
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic Q Science (General)
spellingShingle Q Science (General)
Samsudin, Nurhusna
Hashim, Yumi Zuhanis Has-Yun
Arifin, Mohd Azmir
Mel, Maizirwan
Mohd Salleh, Hamzah
Sopyan, Iis
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
description Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 µg/g on UV/O3 treated microcarriers as compared to the untreated (320 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93o – 49.34o) after UV/O3 treatment and subsequently after immobilisation of gelatin. The attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL microcarrier.
format Conference or Workshop Item
author Samsudin, Nurhusna
Hashim, Yumi Zuhanis Has-Yun
Arifin, Mohd Azmir
Mel, Maizirwan
Mohd Salleh, Hamzah
Sopyan, Iis
author_facet Samsudin, Nurhusna
Hashim, Yumi Zuhanis Has-Yun
Arifin, Mohd Azmir
Mel, Maizirwan
Mohd Salleh, Hamzah
Sopyan, Iis
author_sort Samsudin, Nurhusna
title Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
title_short Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
title_full Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
title_fullStr Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
title_full_unstemmed Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
title_sort surface modification of polycaprolactone (pcl) microcarrier for performance improvement of human skin fibroblast cell culture
publisher Institute of Physics Publishing
publishDate 2018
url http://irep.iium.edu.my/59734/6/59734-Surface%20Modification%20of%20Polycaprolactone.pdf
http://irep.iium.edu.my/59734/12/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29.pdf
http://irep.iium.edu.my/59734/13/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29_SCOPUS.pdf
http://irep.iium.edu.my/59734/
http://iopscience.iop.org/article/10.1088/1757-899X/290/1/012016
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score 13.211869