Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture
Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by...
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my.iium.irep.597342019-10-23T00:39:22Z http://irep.iium.edu.my/59734/ Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Q Science (General) Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 µg/g on UV/O3 treated microcarriers as compared to the untreated (320 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93o – 49.34o) after UV/O3 treatment and subsequently after immobilisation of gelatin. The attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL microcarrier. Institute of Physics Publishing 2018-01-30 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59734/6/59734-Surface%20Modification%20of%20Polycaprolactone.pdf application/pdf en http://irep.iium.edu.my/59734/12/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29.pdf application/pdf en http://irep.iium.edu.my/59734/13/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29_SCOPUS.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd Salleh, Hamzah and Sopyan, Iis (2018) Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture. In: International Conference on Advances in Manufacturing and Materials Engineering 2017 (ICAMME17), 8th-9th August 2017, Kuala Lumpur. http://iopscience.iop.org/article/10.1088/1757-899X/290/1/012016 |
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Q Science (General) Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
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Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work
PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with
solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using
ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL
microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional
barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH)
absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 µg/g on
UV/O3 treated microcarriers as compared to the untreated (320 µg/g) microcarriers. The absorption of
functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated
total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of
hydrophilicity of the surface was confirmed using water contact angle measurement which decreased
(86.93o – 49.34o) after UV/O3 treatment and subsequently after immobilisation of gelatin. The
attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred
much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL
microcarrier. |
format |
Conference or Workshop Item |
author |
Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis |
author_facet |
Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis |
author_sort |
Samsudin, Nurhusna |
title |
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
title_short |
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
title_full |
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
title_fullStr |
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
title_full_unstemmed |
Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture |
title_sort |
surface modification of polycaprolactone (pcl) microcarrier for performance improvement of human skin fibroblast cell culture |
publisher |
Institute of Physics Publishing |
publishDate |
2018 |
url |
http://irep.iium.edu.my/59734/6/59734-Surface%20Modification%20of%20Polycaprolactone.pdf http://irep.iium.edu.my/59734/12/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29.pdf http://irep.iium.edu.my/59734/13/59734_Surface%20Modification%20of%20Polycaprolactone%20%28PCL%29_SCOPUS.pdf http://irep.iium.edu.my/59734/ http://iopscience.iop.org/article/10.1088/1757-899X/290/1/012016 |
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13.211869 |