Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimen...
Saved in:
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
BioMed Central
2017
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf http://irep.iium.edu.my/57413/ https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1777-1?site=bmccomplementalternmed.biomedcentral.com |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.iium.irep.57413 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.574132018-03-09T07:56:23Z http://irep.iium.edu.my/57413/ Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar RC Internal medicine RS Pharmacy and materia medica Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods: Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results: OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions: The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved. BioMed Central 2017-05-25 Article REM application/pdf en http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf application/pdf en http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf Ahmad Azam, Amalina and Pariyani, Raghunath and Ismail, Intan Safinar and Ismail, Amin and Khatib, Alfi and Abas, Faridah and Khozirah, Shaar (2017) Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy. BMC Complementary and Alternative Medicine, 17 (1). pp. 1-13. ISSN 1472-6882 https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1777-1?site=bmccomplementalternmed.biomedcentral.com 10.1186/s12906-017-1777-1 |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English English |
topic |
RC Internal medicine RS Pharmacy and materia medica |
spellingShingle |
RC Internal medicine RS Pharmacy and materia medica Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
description |
Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM).
In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic
protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats.
Methods: Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations.
Results: OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of
OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism.
Conclusions: The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the
biochemical and metabolic mechanisms involved. |
format |
Article |
author |
Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar |
author_facet |
Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar |
author_sort |
Ahmad Azam, Amalina |
title |
Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
title_short |
Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
title_full |
Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
title_fullStr |
Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
title_full_unstemmed |
Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
title_sort |
urinary metabolomics study on the protective role of orthosiphon stamineus in streptozotocin induced diabetes mellitus in rats via 1h nmr spectroscopy |
publisher |
BioMed Central |
publishDate |
2017 |
url |
http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf http://irep.iium.edu.my/57413/ https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1777-1?site=bmccomplementalternmed.biomedcentral.com |
_version_ |
1643615137715716096 |
score |
13.19449 |