Activation of mouse oocytes and in vitro embryo enhancing potential of Nigella Sativa

• Main Authors: Abdul Rahman, Suzanah, Abdul Rahman, Mohd Rahimi Ashraf, Abdul Samat, Nadia Hanis, Basha, Sadak
• Format: Conference or Workshop Item
• Language: English
• Published: 2015
• Subjects: RM300 Drugs and their action
• Online Access: http://irep.iium.edu.my/49392/14/ICAMS%202015%20Programme%20_complete.pdf; http://irep.iium.edu.my/49392/; http://klconference.com/about-conference

Objective: Aim of the present study is to optimize the development of the mouse embryo with addition of different concentrations of Nigella sativa extract in the culture. Methods: In this study, three different concentrations of Nigella sativa supplementation were used to assist in the enhancement of embryo development. G-1 media was used as a control (Group I). Groups II, III and IV were modified with the addition of Nigella sativa at low dose (5%), moderate dose (10%) and high dose (15%) respectively. Results: Results showed an increase in the number of oocytes that successfully developed into morula and blastocyst after supplementation with three different concentrations of Nigella sativa in the embryo medium. Conclusions: The outcome of the study indicated that Nigella sativa supplementation at a moderate concentration may help in the optimization of mouse embryo culture leading to an increase in the number of morulas and blastocysts.