Scaling-up recombinant enzyme fermentation

• Main Authors: Azmi, Azlin Suhaida, Sulaiman, Sarina, Mohamed Azmin, Nor Fadhillah, Ali, Fathilah
• Other Authors: Amid, Azura
• Format: Book Chapter
• Language: English
• Published: Springer International Publishing 2015
• Subjects: TA164 Bioengineering
• Online Access: http://irep.iium.edu.my/47856/8/One-Kenobi.pdf; http://irep.iium.edu.my/47856/; http://link.springer.com/chapter/10.1007%2F978-3-319-12397-4_7

Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed.