Scaling-up recombinant enzyme fermentation
Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodol...
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| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Book Chapter |
| Language: | English |
| Published: |
Springer International Publishing
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47856/8/One-Kenobi.pdf http://irep.iium.edu.my/47856/ http://link.springer.com/chapter/10.1007%2F978-3-319-12397-4_7 |
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| Summary: | Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed. |
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