A novel animal model for subcutaneous soft tissue infection
In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Metho...
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2014
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Online Access: | http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf http://irep.iium.edu.my/44152/ https://www.jstage.jst.go.jp/article/jsmbe/52/Supplement/52_O-285/_article http://doi.org/10.11239/jsmbe.52.O-285 |
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my.iium.irep.441522015-08-18T00:20:18Z http://irep.iium.edu.my/44152/ A novel animal model for subcutaneous soft tissue infection Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto RZ Other systems of medicine TP248.13 Biotechnology In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence. Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections. J-STAGE 2014 Article REM application/pdf en http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf Zulaziz, Natasha and N. , Himeno and H. , Miyazaki and D. , Saitoh and Noor Azmi, Azran Azhim and Y. , Morimoto (2014) A novel animal model for subcutaneous soft tissue infection. Biomedical Engineering, 52. pp. 285-286. ISSN 1881-4379 https://www.jstage.jst.go.jp/article/jsmbe/52/Supplement/52_O-285/_article http://doi.org/10.11239/jsmbe.52.O-285 |
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RZ Other systems of medicine TP248.13 Biotechnology Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto A novel animal model for subcutaneous soft tissue infection |
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In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed.
Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence.
Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.
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Article |
author |
Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto |
author_facet |
Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto |
author_sort |
Zulaziz, Natasha |
title |
A novel animal model for subcutaneous soft tissue infection |
title_short |
A novel animal model for subcutaneous soft tissue infection |
title_full |
A novel animal model for subcutaneous soft tissue infection |
title_fullStr |
A novel animal model for subcutaneous soft tissue infection |
title_full_unstemmed |
A novel animal model for subcutaneous soft tissue infection |
title_sort |
novel animal model for subcutaneous soft tissue infection |
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J-STAGE |
publishDate |
2014 |
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http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf http://irep.iium.edu.my/44152/ https://www.jstage.jst.go.jp/article/jsmbe/52/Supplement/52_O-285/_article http://doi.org/10.11239/jsmbe.52.O-285 |
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13.18916 |