A novel animal model for subcutaneous soft tissue infection

• Main Authors: Zulaziz, Natasha, N. , Himeno, H. , Miyazaki, D. , Saitoh, Noor Azmi, Azran Azhim, Y. , Morimoto
• Format: Article
• Language: English
• Published: J-STAGE 2014
• Subjects: RZ Other systems of medicine; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf; http://irep.iium.edu.my/44152/; https://www.jstage.jst.go.jp/article/jsmbe/52/Supplement/52_O-285/_article; http://doi.org/10.11239/jsmbe.52.O-285

In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence. Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.