Optimization of newcastle disease virus production in t-flask
In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on viru...
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2011
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| Online Access: | http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf http://irep.iium.edu.my/3643/ http://www.iium.edu.my/icbioe/2011/ |
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my.iium.irep.36432012-01-19T00:25:56Z http://irep.iium.edu.my/3643/ Optimization of newcastle disease virus production in t-flask Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah QR355 Virology In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. 2011-05-17 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf Arifin, Mohd Azmir and Salim, Siti Hajar and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Syed Hassan, Sharifah (2011) Optimization of newcastle disease virus production in t-flask. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
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QR355 Virology |
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QR355 Virology Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah Optimization of newcastle disease virus production in t-flask |
| description |
In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. |
| format |
Conference or Workshop Item |
| author |
Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah |
| author_facet |
Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah |
| author_sort |
Arifin, Mohd Azmir |
| title |
Optimization of newcastle disease virus production in t-flask |
| title_short |
Optimization of newcastle disease virus production in t-flask |
| title_full |
Optimization of newcastle disease virus production in t-flask |
| title_fullStr |
Optimization of newcastle disease virus production in t-flask |
| title_full_unstemmed |
Optimization of newcastle disease virus production in t-flask |
| title_sort |
optimization of newcastle disease virus production in t-flask |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf http://irep.iium.edu.my/3643/ http://www.iium.edu.my/icbioe/2011/ |
| _version_ |
1643605182159781888 |
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13.209306 |