Optimization of newcastle disease virus production in t-flask
In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on viru...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf http://irep.iium.edu.my/3643/ http://www.iium.edu.my/icbioe/2011/ |
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| Summary: | In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. |
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