Production of alpha-amylase by immobolized bacillus subtilis in polymeric polyhipe matrix
The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. A suspension of B. subtilis spores were inoculated into a synthesized PHP matrix whic...
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/27372/1/Revised_Paper_dzun_ICCBPE_2012.pdf http://irep.iium.edu.my/27372/ |
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| Summary: | The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. A suspension of B. subtilis spores were inoculated into a synthesized PHP matrix which chemically modified and this PHP matrix was named sulphonated PHPs (SPHPs). These inoculated spores were then activated by supplying continuously well-aerated culture medium (LB medium) and placed in a 370C room for 24h incubation. The growth and enzyme productivity data were evaluated and compared. Three different pore and interconnect sizes of SPHPs were evaluated; 42.0 |
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