Protease purification from Bacillus amyloliquefaciens B7 using Aqueous Two-Phase System (ATPS)

• Main Authors: Abd Samad, Nadiah Syuhada, Amid, Azura, Jimat, Dzun Noraini, Ab Shukor, Nurul Aqilah
• Format: Article
• Language: English
• Published: Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/59487/1/59487_Protease%20purification%20from%20Bacillus.pdf; http://irep.iium.edu.my/59487/; http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(6)%20R1.pdf

Bacillus amyloliquefaciens B7 was isolated from the fermented fish sauce and identified as protease producer. Generally, in downstream processing, purification of enzymes consumes higher cost regarding reagents and equipment used. Moreover, harsh purification methods used might cause denaturation of...