Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase
This study concerns with the production and partial characterization of alkaline cellulase from alkaliphilic cellulolytic (AC) fungi isolated fromsoil in Perlis, Malaysia. The best fungi strain was selected on the basis of producing the highest cellulase at high pH conditions. Cellulase from th...
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De Gruyter
2024
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my.iium.irep.1132982024-07-25T07:14:53Z http://irep.iium.edu.my/113298/ Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase Zainuddin, Nor'Izzah Mohd Zaini Makhtar, Muaz Nagoor Gunny, Ahmad Anas Subash Chandra Bose, Gopinath Ahmad, Abdul Aziz Pusphanathan, Kavita Siddiqui, Masoom Raza Alam, Mahboob Mohd, Rafatullah Q Science (General) QD Chemistry QR Microbiology This study concerns with the production and partial characterization of alkaline cellulase from alkaliphilic cellulolytic (AC) fungi isolated fromsoil in Perlis, Malaysia. The best fungi strain was selected on the basis of producing the highest cellulase at high pH conditions. Cellulase from the selected fungi strain was further characterized under saccharification but varies in operating parameters. Finally, the kineticmodel describing the growth of the AC fungi strain was studied by employing the logistic model. Among the tested fungi strains, Basidiomycetes strain (BK1) showed high potentiality for the production of maximum alkaline cellulase production at pH 9 after 72 h of incubation at 30°C containing 6 g·L−1 carboxyl methyl cellulose. The saccharification process showed that the enzyme favour high alkaline condition and proves thermotolerant properties, while 15% (v/v) enzyme loading and 1% substrate concentration recorded the highest glucose production at about 1.2–1.3mg·mL−1. The novelty of the study is to identify and optimize a unique indigenous fungi that emit alkaliphilic cellulase as alternative usage in biotechnology industries due to its capacity to adapt to the extreme conditions of specific industrial processes. There are revolutionary options for use in biotechnological businesses that involve high pH and therefore have substantial biotechnological promise. De Gruyter 2024-02-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113298/7/113298_%20Isolation%2C%20screening%20and%20optimization.pdf application/pdf en http://irep.iium.edu.my/113298/8/113298_%20Isolation%2C%20screening%20and%20optimization_Scopus.pdf Zainuddin, Nor'Izzah and Mohd Zaini Makhtar, Muaz and Nagoor Gunny, Ahmad Anas and Subash Chandra Bose, Gopinath and Ahmad, Abdul Aziz and Pusphanathan, Kavita and Siddiqui, Masoom Raza and Alam, Mahboob and Mohd, Rafatullah (2024) Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase. Green Processing and Synthesis, 13 (1). pp. 1-16. ISSN 2191-9550 https://www.degruyter.com/document/doi/10.1515/gps-2023-0153/html https://doi.org/10.1515/gps-2023-0153 |
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Q Science (General) QD Chemistry QR Microbiology Zainuddin, Nor'Izzah Mohd Zaini Makhtar, Muaz Nagoor Gunny, Ahmad Anas Subash Chandra Bose, Gopinath Ahmad, Abdul Aziz Pusphanathan, Kavita Siddiqui, Masoom Raza Alam, Mahboob Mohd, Rafatullah Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase |
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This study concerns with the production and
partial characterization of alkaline cellulase from alkaliphilic
cellulolytic (AC) fungi isolated fromsoil in Perlis,
Malaysia. The best fungi strain was selected on the basis
of producing the highest cellulase at high pH conditions.
Cellulase from the selected fungi strain was further characterized
under saccharification but varies in operating
parameters. Finally, the kineticmodel describing the growth
of the AC fungi strain was studied by employing the logistic
model. Among the tested fungi strains, Basidiomycetes strain
(BK1) showed high potentiality for the production of maximum
alkaline cellulase production at pH 9 after 72 h of
incubation at 30°C containing 6 g·L−1 carboxyl methyl cellulose.
The saccharification process showed that the enzyme
favour high alkaline condition and proves thermotolerant
properties, while 15% (v/v) enzyme loading and 1% substrate
concentration recorded the highest glucose production at
about 1.2–1.3mg·mL−1. The novelty of the study is to identify
and optimize a unique indigenous fungi that emit alkaliphilic
cellulase as alternative usage in biotechnology industries
due to its capacity to adapt to the extreme conditions
of specific industrial processes. There are revolutionary
options for use in biotechnological businesses that involve
high pH and therefore have substantial biotechnological
promise. |
format |
Article |
author |
Zainuddin, Nor'Izzah Mohd Zaini Makhtar, Muaz Nagoor Gunny, Ahmad Anas Subash Chandra Bose, Gopinath Ahmad, Abdul Aziz Pusphanathan, Kavita Siddiqui, Masoom Raza Alam, Mahboob Mohd, Rafatullah |
author_facet |
Zainuddin, Nor'Izzah Mohd Zaini Makhtar, Muaz Nagoor Gunny, Ahmad Anas Subash Chandra Bose, Gopinath Ahmad, Abdul Aziz Pusphanathan, Kavita Siddiqui, Masoom Raza Alam, Mahboob Mohd, Rafatullah |
author_sort |
Zainuddin, Nor'Izzah |
title |
Isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
title_short |
Isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
title_full |
Isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
title_fullStr |
Isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
title_full_unstemmed |
Isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
title_sort |
isolation, screening and optimization
of alkaliphilic cellulolytic fungi for
production of cellulase |
publisher |
De Gruyter |
publishDate |
2024 |
url |
http://irep.iium.edu.my/113298/7/113298_%20Isolation%2C%20screening%20and%20optimization.pdf http://irep.iium.edu.my/113298/8/113298_%20Isolation%2C%20screening%20and%20optimization_Scopus.pdf http://irep.iium.edu.my/113298/ https://www.degruyter.com/document/doi/10.1515/gps-2023-0153/html https://doi.org/10.1515/gps-2023-0153 |
_version_ |
1805880578263744512 |
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13.188404 |