Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase
This study concerns with the production and partial characterization of alkaline cellulase from alkaliphilic cellulolytic (AC) fungi isolated fromsoil in Perlis, Malaysia. The best fungi strain was selected on the basis of producing the highest cellulase at high pH conditions. Cellulase from th...
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Main Authors: | , , , , , , , , |
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Format: | Article |
Language: | English English |
Published: |
De Gruyter
2024
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Subjects: | |
Online Access: | http://irep.iium.edu.my/113298/7/113298_%20Isolation%2C%20screening%20and%20optimization.pdf http://irep.iium.edu.my/113298/8/113298_%20Isolation%2C%20screening%20and%20optimization_Scopus.pdf http://irep.iium.edu.my/113298/ https://www.degruyter.com/document/doi/10.1515/gps-2023-0153/html https://doi.org/10.1515/gps-2023-0153 |
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Summary: | This study concerns with the production and
partial characterization of alkaline cellulase from alkaliphilic
cellulolytic (AC) fungi isolated fromsoil in Perlis,
Malaysia. The best fungi strain was selected on the basis
of producing the highest cellulase at high pH conditions.
Cellulase from the selected fungi strain was further characterized
under saccharification but varies in operating
parameters. Finally, the kineticmodel describing the growth
of the AC fungi strain was studied by employing the logistic
model. Among the tested fungi strains, Basidiomycetes strain
(BK1) showed high potentiality for the production of maximum
alkaline cellulase production at pH 9 after 72 h of
incubation at 30°C containing 6 g·L−1 carboxyl methyl cellulose.
The saccharification process showed that the enzyme
favour high alkaline condition and proves thermotolerant
properties, while 15% (v/v) enzyme loading and 1% substrate
concentration recorded the highest glucose production at
about 1.2–1.3mg·mL−1. The novelty of the study is to identify
and optimize a unique indigenous fungi that emit alkaliphilic
cellulase as alternative usage in biotechnology industries
due to its capacity to adapt to the extreme conditions
of specific industrial processes. There are revolutionary
options for use in biotechnological businesses that involve
high pH and therefore have substantial biotechnological
promise. |
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