Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry
The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (R...
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MYU K.K.
2023
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my.iium.irep.1080842024-02-02T08:27:19Z http://irep.iium.edu.my/108084/ Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry Zulhairee, Munirah Mohd Zain, Rozainanee Che Mohamad Nor, Adibah Jafar, Nur Fadhilah Md Noh, Mohd Fairulnizal Ahmad Noorden, Mohd Shihabuddin Nordin, Anis Nurashikin Ab Rahim, Rosminazuin Gunawan, Teddy Surya Chin, Lim Ying Mohd, Yusairie Azman, Nur Asyiqin Mohd Zain, Zainiharyati TK7885 Computer engineering The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay on gold-nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) using differential pulse voltammetry (DPV) and to compare it with real-time RT-PCR. The shape of the electrodeposited AuNP on SPCE was quasi-spherical with a size of ±500 nm. The developed RT-LAMP primer was designed from the GenBank database using the NCBI Multiple Alignment tools and Jalview software. Nasopharyngeal clinical samples were obtained from suspected COVID-19 patients (n = 148). The RT-LAMP products were dropped on the modified AuNP/SPCE under DPV setting, which resulted in current change (∆I) responses. The positive and negative samples produced significantly different ∆I signals with a p-value <0.0001 at a 95% confidence interval using Student’s t-test. The RT-LAMP assay using Au/SPCE exhibited a 30 s response time per analysis. The clinical sensitivity and specificity obtained were 79.7% and 85.1%, respectively, with a detection limit of 0.4 copies µl−1. Hence, this proposed method is suitable for COVID-19 RNA detection in resource-limited settings. MYU K.K. 2023-10-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/108084/7/108084-Clinical%20performance%20of%20reverse%20transcription.pdf application/pdf en http://irep.iium.edu.my/108084/13/108084_Clinical%20performance%20of%20reverse%20transcription_Scopus.pdf Zulhairee, Munirah and Mohd Zain, Rozainanee and Che Mohamad Nor, Adibah and Jafar, Nur Fadhilah and Md Noh, Mohd Fairulnizal and Ahmad Noorden, Mohd Shihabuddin and Nordin, Anis Nurashikin and Ab Rahim, Rosminazuin and Gunawan, Teddy Surya and Chin, Lim Ying and Mohd, Yusairie and Azman, Nur Asyiqin and Mohd Zain, Zainiharyati (2023) Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry. Sensors and Materials, 35 (10). pp. 4731-4750. ISSN 0914-4935 E-ISSN 2435-0869 https://sensors.myu-group.co.jp/article.php?ss=4430 https://doi.org/10.18494/SAM4430 |
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TK7885 Computer engineering Zulhairee, Munirah Mohd Zain, Rozainanee Che Mohamad Nor, Adibah Jafar, Nur Fadhilah Md Noh, Mohd Fairulnizal Ahmad Noorden, Mohd Shihabuddin Nordin, Anis Nurashikin Ab Rahim, Rosminazuin Gunawan, Teddy Surya Chin, Lim Ying Mohd, Yusairie Azman, Nur Asyiqin Mohd Zain, Zainiharyati Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
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The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay on gold-nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) using differential pulse voltammetry (DPV) and to compare it with real-time RT-PCR. The shape of the electrodeposited AuNP on SPCE was quasi-spherical with a size of ±500 nm. The developed RT-LAMP primer was designed from the GenBank database using the NCBI Multiple Alignment tools and Jalview software. Nasopharyngeal clinical samples were obtained from suspected COVID-19 patients (n = 148). The RT-LAMP products were dropped on the modified AuNP/SPCE under DPV setting, which resulted in current change (∆I) responses. The positive and negative samples produced significantly different ∆I signals with a p-value <0.0001 at a 95% confidence interval using Student’s t-test. The RT-LAMP assay using Au/SPCE exhibited a 30 s response time per analysis. The clinical sensitivity and specificity obtained were 79.7% and 85.1%, respectively, with a detection limit of 0.4 copies µl−1. Hence, this proposed method is suitable for COVID-19 RNA detection in resource-limited settings. |
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Article |
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Zulhairee, Munirah Mohd Zain, Rozainanee Che Mohamad Nor, Adibah Jafar, Nur Fadhilah Md Noh, Mohd Fairulnizal Ahmad Noorden, Mohd Shihabuddin Nordin, Anis Nurashikin Ab Rahim, Rosminazuin Gunawan, Teddy Surya Chin, Lim Ying Mohd, Yusairie Azman, Nur Asyiqin Mohd Zain, Zainiharyati |
author_facet |
Zulhairee, Munirah Mohd Zain, Rozainanee Che Mohamad Nor, Adibah Jafar, Nur Fadhilah Md Noh, Mohd Fairulnizal Ahmad Noorden, Mohd Shihabuddin Nordin, Anis Nurashikin Ab Rahim, Rosminazuin Gunawan, Teddy Surya Chin, Lim Ying Mohd, Yusairie Azman, Nur Asyiqin Mohd Zain, Zainiharyati |
author_sort |
Zulhairee, Munirah |
title |
Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
title_short |
Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
title_full |
Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
title_fullStr |
Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
title_full_unstemmed |
Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry |
title_sort |
clinical performance of reverse transcription loop mediated isothermal amplification covid-19 assay on gold- nanoparticle-modified screen-printed carbon electrode using differential pulse voltammetry |
publisher |
MYU K.K. |
publishDate |
2023 |
url |
http://irep.iium.edu.my/108084/7/108084-Clinical%20performance%20of%20reverse%20transcription.pdf http://irep.iium.edu.my/108084/13/108084_Clinical%20performance%20of%20reverse%20transcription_Scopus.pdf http://irep.iium.edu.my/108084/ https://sensors.myu-group.co.jp/article.php?ss=4430 https://doi.org/10.18494/SAM4430 |
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1789940149600124928 |
score |
13.160551 |