Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry
The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (R...
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Main Authors: | , , , , , , , , , , , , |
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Format: | Article |
Language: | English English |
Published: |
MYU K.K.
2023
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Online Access: | http://irep.iium.edu.my/108084/7/108084-Clinical%20performance%20of%20reverse%20transcription.pdf http://irep.iium.edu.my/108084/13/108084_Clinical%20performance%20of%20reverse%20transcription_Scopus.pdf http://irep.iium.edu.my/108084/ https://sensors.myu-group.co.jp/article.php?ss=4430 https://doi.org/10.18494/SAM4430 |
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Summary: | The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay on gold-nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) using differential pulse voltammetry (DPV) and to compare it with real-time RT-PCR. The shape of the electrodeposited AuNP on SPCE was quasi-spherical with a size of ±500 nm. The developed RT-LAMP primer was designed from the GenBank database using the NCBI Multiple Alignment tools and Jalview software. Nasopharyngeal clinical samples were obtained from suspected COVID-19 patients (n = 148). The RT-LAMP products were dropped on the modified AuNP/SPCE under DPV setting, which resulted in current change (∆I) responses. The positive and negative samples produced significantly different ∆I signals with a p-value <0.0001 at a 95% confidence interval using Student’s t-test. The RT-LAMP assay using Au/SPCE exhibited a 30 s response time per analysis. The clinical sensitivity and specificity obtained were 79.7% and 85.1%, respectively, with a detection limit of 0.4 copies µl−1. Hence, this proposed method is suitable for COVID-19 RNA detection in resource-limited settings. |
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