Comparison on effects of temperature on different strains of phytase producing bacteria isolated from Malaysia’s hot spring
The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia, which...
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Format: | Article |
Language: | English English |
Published: |
Penerbit Universiti Sultan Zainal Abidin
2020
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Subjects: | |
Online Access: | http://eprints.unisza.edu.my/7287/1/FH02-PUSPA-20-43608.pdf http://eprints.unisza.edu.my/7287/2/FH02-PUSPA-21-51313.pdf http://eprints.unisza.edu.my/7287/ |
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Summary: | The main purpose of this research was to find the best growth curve for bacterial growth and the optimum
temperature for the production of phytase from different potential phytase producing bacterial strains. A total
of four strains used were originally isolated from hot springs in Malaysia, which were in Labis, Johor (L3), Dusun
Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). Nutrient Agar (NA) and modified Phytase
Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out
through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively
measured at different temperatures at (30°C and 37°C) values. Enzyme activity was determined according to
the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/
mL). As for the overall, strain L3 (from Labis, Johor) exhibit promising rate of Pi released in the media at 30°C
and 37°C, with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL, respectively. The pH of
the cultures was also measured, where it shows that strains grown in cultures at 37°C produced a higher phytase
activity and resulting a lower reading of pH compared when grown at 30°C. All around, L3 strains has the lowest
value of pH when cultured at 30°C and 37°C, with the pH value of 3.62 and 2.37, respectively. From the result
obtained, the lower pH indicates the process of phytic acid degradation take place by the phytase in producing
inorganic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally
taken from Hot Springs, further analysis of temperature optimization using 55°C and even 60°C should be
carried out. In the future, biochemical research and molecular identification may also be carried out to identify
molecular identity in the strains. |
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