Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing...
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my-unisza-ir.63632022-05-22T02:50:26Z http://eprints.unisza.edu.my/6363/ Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis Mohd Hilmi, Abu Bakar Mohammad Abdulraheem, AL-kafaween Rao Sanaullah, Khan QH301 Biology Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing the bound dye with 33% glacial acetic acid, and measuring the optical density (OD) of the solution at 590 nm by using a microplate reader. Results: It was found that 3 and 5 days of incubation are critical for biofilm formation as indicated by the OD values of 0.55 - 0.06 and 0.70 - 0.39 in MHB and TSB respectively at OD 0.05. Similar results were noted for OD 0.1 in both media MHB and TSB while TSB showing slightly better results for biofilm formation. Conclusion: It is concluded that three days (72 hours) are required for obtaining effective biofilm formation in both MHB and TSB at 370C at OD 0.05 and OD 0.1. 2019-03 Article PeerReviewed text en http://eprints.unisza.edu.my/6363/1/FH02-FSK-19-28688.pdf Mohd Hilmi, Abu Bakar and Mohammad Abdulraheem, AL-kafaween and Rao Sanaullah, Khan (2019) Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis. EC Microbiology, 15 (4). pp. 277-282. |
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QH301 Biology Mohd Hilmi, Abu Bakar Mohammad Abdulraheem, AL-kafaween Rao Sanaullah, Khan Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
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Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing
the bound dye with 33% glacial acetic acid, and measuring the optical density (OD) of the solution at 590 nm by using a microplate
reader. Results: It was found that 3 and 5 days of incubation are critical for biofilm formation as indicated by the OD values of 0.55 - 0.06
and 0.70 - 0.39 in MHB and TSB respectively at OD 0.05. Similar results were noted for OD 0.1 in both media MHB and TSB while TSB
showing slightly better results for biofilm formation. Conclusion: It is concluded that three days (72 hours) are required for obtaining effective biofilm formation in both MHB and TSB
at 370C at OD 0.05 and OD 0.1. |
format |
Article |
author |
Mohd Hilmi, Abu Bakar Mohammad Abdulraheem, AL-kafaween Rao Sanaullah, Khan |
author_facet |
Mohd Hilmi, Abu Bakar Mohammad Abdulraheem, AL-kafaween Rao Sanaullah, Khan |
author_sort |
Mohd Hilmi, Abu Bakar |
title |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
title_short |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
title_full |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
title_fullStr |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
title_full_unstemmed |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis |
title_sort |
effect of growth media and optical density on biofilm formation by staphylococcus epidermidis |
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2019 |
url |
http://eprints.unisza.edu.my/6363/1/FH02-FSK-19-28688.pdf http://eprints.unisza.edu.my/6363/ |
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