Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/6363/1/FH02-FSK-19-28688.pdf http://eprints.unisza.edu.my/6363/ |
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| Summary: | Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing
the bound dye with 33% glacial acetic acid, and measuring the optical density (OD) of the solution at 590 nm by using a microplate
reader. Results: It was found that 3 and 5 days of incubation are critical for biofilm formation as indicated by the OD values of 0.55 - 0.06
and 0.70 - 0.39 in MHB and TSB respectively at OD 0.05. Similar results were noted for OD 0.1 in both media MHB and TSB while TSB
showing slightly better results for biofilm formation. Conclusion: It is concluded that three days (72 hours) are required for obtaining effective biofilm formation in both MHB and TSB
at 370C at OD 0.05 and OD 0.1. |
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