Antioxidant, antimicrobial activity and in-vitro cytotoxicity screening study of pili nut oil
Some vegetable oils contain natural antioxidants such as beta carotene and vitamin E namely alpha tocopherol. The objective of this study was to screening the value of α-tocopherol, β-carotene, antioxidant capacity, antimicrobial activity and toxicological properties of roasted pili nut oil (RPNO...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/5052/1/FH02-FPBSM-14-00751.jpg http://eprints.unisza.edu.my/5052/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Some vegetable oils contain natural antioxidants such as beta carotene and vitamin E namely
alpha tocopherol. The objective of this study was to screening the value of α-tocopherol,
β-carotene, antioxidant capacity, antimicrobial activity and toxicological properties of roasted
pili nut oil (RPNO) and unroasted pili nut oil (UPNO). The result showed that RPNO contained
higher amount of vitamin E and less amount of beta carotene compared to UPNO. RPNO
and UPNO scavenged DPPH radicals by 24.66% and 9.52% at concentration of 140 µg/ml.
The total phenolic compound (TPC) in UPNO and RPNO were about 19.96 ± 0.52 mg/kg
and 12.43 ± 0.69 mg/kg respectively. It was observed that bacteria species exhibited different
sensitivities towards RPNO, UPNO, Gentamycin, Ampicillin and Chloramphenicol. Bacillus
cereus 14570 was the most sensitive bacterium and all strains of Staphylococcus aureus tested
were resistant against both samples RPNO and UPNO. An in vitro toxicological study based
on the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cytotoxicity
assay was also performed. In vitro cytotoxicity indicated that both RPNO and UPNO had no
effect against HeLa (cervical cancer cell), MCF-7 (breast cancer cell) and HT-29 (human colon
adenocarcinoma cell) cell lines tested. |
|---|