Carbon sources effects on different strains of phytase producing bacteria isolated from Malaysia’s hot spring

• Main Authors: Nurul Asma Hasliza, Zulkifly, Nurul Izyan, Che Mohamood
• Format: Conference or Workshop Item
• Language: English
• Published: 2021
• Subjects: QR Microbiology; S Agriculture (General)
• Online Access: http://eprints.unisza.edu.my/4253/1/FH03-FBIM-21-55808.pdf; http://eprints.unisza.edu.my/4253/

This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD), colony forming unit (CFU), and their phytase production. All four strains of bacteria potentially producing phytase have been isolated from several hot springs in Malaysia, which were in Labis, Johor (L3), Dusun Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). The bacteria were grown in nutrient agar (NA) and modified Phytase Screening Medium (PSM) liquid media for culture enrichment. Glucose and lactose were used as the carbon source and growth was done under optimum culture conditions (pH 5.5, 37˚C, 200 rpm) for 72 hours through batch culture method using a shake-flask scale. All experiments were done in triplicates. For quantitative screening of phytase production, the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources, glucose have shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile, the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.040 U/mL), RT (0.036 U/mL), B9 (0.026 U/mL), and A (0.026 U/mL). As for the overall, strain L3 (from Labis, Johor) gave promising rate of inorganic phosphate released with optimum phytase activity value of 0.04 U/mL in presence of glucose and lactose. In the future, biochemical research and molecular identification may also be carried out to identify molecular identity in the strains. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo – inositol phosphates that is believed to have important pharmacological effects.